Table 2 Primers used for reverse transcription quantitative PCR.
From: TNF-α- and tumor-induced skeletal muscle atrophy involves sphingolipid metabolism
Name | Direction | Sequence 5'→3' |
|---|---|---|
PLD1 | Sense | GGTCAGAAAGATAACCCAGG |
Anti-sense | GAAGCGAGACAGCGAAATGG | |
Atrogin-1 | Sense | CTCTGCCAGTACCACTTCTC |
Anti-sense | ATGGTCAGTGCCCCTCCAGG | |
LC3b | Sense | CTGGACAAGACCAAGTTCCT |
Anti-sense | AAGCCGTCTTCATCTCTCTC | |
S6K1 | Sense | AGAGCACCTGCGTATGAATC |
Anti-sense | CACTGACTCTTTGAGACTGCC | |
Murf1 | Sense | TGCATCTCCATGCTGGTGGC |
Anti-sense | CTTCTTCTCGTCCAGGATGG | |
Foxo1a | Sense | AGATCTACGAGTGGATGGTG |
Anti-sense | GGACAGATTGTGGCGAATTG | |
Foxo3a | Sense | GAGAGCAGATTTGGCAAAGG |
Anti-sense | CCTCATCTCCACACAGAACG | |
TBP | Sense | TGGTGTGCACAGGAGCCAAG |
Anti-sense | TTCACATCACAGCTCCCCAC | |
Cyclophilin A | Sense | ATGGCACTGGTGGCAAGTCC |
Anti-sense | TTGCCATTCCTGGACCCAAA |