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Figure 2 | Skeletal Muscle

Figure 2

From: p38β MAPK upregulates atrogin1/MAFbx by specific phosphorylation of C/EBPβ

Figure 2

Expression of constitutively active p38β in C2C12 myotubes resulted in specific phosphorylation of C/EBPβ at Thr-188 and upregulation of atrogin1/MAFbx. C2C12 myoblasts were transfected with a plasmid encoding constitutively active p38α, p38β or the empty vector (control). The myoblasts were allowed to differentiate for 96 h to form myotubes that were then harvested and analyzed for ATF2 activation, Thr-188 phosphorylation in C/EBPβ and level of atrogin1/MAFbx using western blotting.

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