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Figure 1 | Skeletal Muscle

Figure 1

From: A role for RNA post-transcriptional regulation in satellite cell activation

Figure 1

Sdc4−/−satellite cell gene expression post-muscle injury is similar to freshly isolated satellite cells. Myofiber-associated satellite cells are immunoreactive for MyoD 24 h and 48 h after isolation from wild type mice but not Sdc4−/− mice (A, B). Wild type but not Sdc4−/− cells divide by 48 h in culture (B) where c-met (red), MyoD (green), and DAPI (blue) identify satellite cells and a dashed line indicates the position of the myofiber membrane (A, B). Flow cytometry histograms of wild type (C) and syndecan-4 null (D) mononuclear cells from uninjured and injured skeletal muscle 12 h and 48 h post-injury plotted for cell size (FSC) vs. internal complexity (SSC), where the red box indicates gating for further analysis to remove debris (upper panels). Syndecan-3 immunoreactive cells present in the gate were isolated from wild type mice (C, lower panel) and Sdc4−/− mice (D, lower panel) where the percentages indicate satellite cells (blue lines) relative to other events with false-positives set to an antibody background < 0.1% (red lines). A hierarchical dendrogram constructed with Spotfire™ DecisionSite using Affymetrix GeneChip data reveals that Sdc4−/− satellite cells cluster most closely to freshly isolated wild type satellite cells while injured wild type satellite cells either 12 h post-injury or 48 h post-injury cluster independently (E). Red depicts high relative gene expression and green depicts low relative expression in the hierarchical cluster dendrograms (UPGMA, Euclidean distance). FI, freshly isolated; PI, post-injury.

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