Figure 8

Candidate miRNAs target genes involved in cell growth, survival, migration, and cell cycle progression. The predicted target genes of miR-16, miR-93, miR-106b, and miR-124 were identified using Ingenuity® Systems (http://www.ingenuity.com). PTEN Signaling and Cell Cycle Regulation by BTG Family Proteins emerged as the top ranked canonical pathway regulated during satellite cell activation. The pathways and relative expression changes occurring during satellite cell activation are depicted. Note that the miRNAs have opposite expression profiles of their respective target mRNAs. Red indicates mRNAs downregulated and green indicates mRNAs upregulated with the intensity of red or green indicating increasing or decreasing fold change, respectively. The data compare mRNAs unique to wild type satellite cells isolated 12 h post-injury to satellite cells isolated from uninjured skeletal muscle.