Figure 1

Canine muscle cell engraftment into mouse muscle is quantifiable and consistent. (A) Cryosections from NOD/SCID mouse muscle injected with 1 × 10⁴ (a, b) or 4 × 10⁴ (c, d) canine muscle-derived mononuclear cells were immunostained with anti-lamin A/C (a, c), or anti-dystrophin (MANDYS107 - b, d) and fluorescently labeled secondary antibodies. (B, C) Cryosections from mouse muscle injected with canine muscle-derived mononuclear cells were immunostained with anti-lamin A/C or anti-dystrophin (MANDYS107), and fluorescently labeled secondary antibodies. The number of fibers expressing canine dystrophin (B) and the number of nuclei expressing canine lamin A/C (C) per cross-section were counted using cryosections surrounding the region of highest engraftment within the muscle. The points on the graphs represent the average of the averages ± SD, where the average was calculated from three cryosections per mouse, and the average of the averages was calculated from at least three mice per cell dose, and at least two separate cell isolations per cell dose. (D) The number of nuclei expressing canine lamin A/C was plotted as a function of the number of fibers expressing canine dystrophin per cross-section.