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Figure 1 | Skeletal Muscle

Figure 1

From: A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts

Figure 1

FSBA is a pan-kinase inhibitor that allows for kinase-specific substrate labelling of cell lysate. (A) C2C12 cell lysate was treated with either nothing (lane 1), dimethyl sulphoxide (DMSO) or 5'-4-fluorosulphonylbenzoyladenosine (FSBA) solubilised in DMSO. Subsequently, samples were desalted, kinase assay buffer containing 32P-γ-ATP was added and the samples were assayed for 1.5 hours. Treatment with FSBA abolished the labelling of endogenous protein kinase substrates. (B) After pretreatment of C2C12 cell lysate with FSBA, purified p38α was added with a kinase assay buffer to specifically label its substrates and visualised using one-dimensional SDS-PAGE or two-dimensional gel electrophoresis (Additional file 1 Figure S1).

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