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Figure 1 | Skeletal Muscle

Figure 1

From: Retracted: MyoD-dependent regulation of NF-κB activity couples cell-cycle withdrawal to myogenic differentiation

Figure 1

NF-κB remains localized to the nucleus after serum withdrawal in MyoD-/-myoblasts. (A) Protein was isolated from a differentiation time course of WT and MyoD-/- primary myoblasts and a western blot using the nuclear (N) and cytoplasmic (C) fractions was performed with an antibody specific for the p65 subunit of NF-κB. A western blot with an antibody specific for α-tubulin was performed using the cytoplasmic fractions. (B) Immunofluorescence analysis of WT and MyoD-/- myoblasts and myotubes demonstrate that NF-κB remains localized in the nucleus in MyoD-/- myoblasts even after differentiation. Staining for NF-κB in red, nuclei are counterstained with DAPI (in blue). Scale bar: 20 μm.

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