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Figure 2 | Skeletal Muscle

Figure 2

From: Retracted: MyoD-dependent regulation of NF-κB activity couples cell-cycle withdrawal to myogenic differentiation

Figure 2

Loss of MyoD activates NF-κB. (A) Protein isolated from C2C12 myoblasts transfected with control siRNA or siRNA specific for MyoD were analyzed by western blot using antibodies specific to MyoD and α-tubulin. (B) C2C12 cells transfected with control siRNA (siCont) or MyoD siRNA (siMyoD) were co-transfected with an NF-kB reporter vector (3xκB-Luc). Bars represent average luciferase activity (relative light units (RLUs)) (n = 3). Error bars represent standard deviation. (C) Same as B, with the addition of a p65 (NF-κB) expression vector.

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Skeletal Muscle

ISSN: 2044-5040

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