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Figure 3 | Skeletal Muscle

Figure 3

From: miR-206 integrates multiple components of differentiation pathways to control the transition from growth to differentiation in rhabdomyosarcoma cells

Figure 3

ZNF238 is a downstream target of MyoD and RUNX1. (A) qPCR for RUNX1 and ZNF238 in RD cells transduced with virus expressing the converse factor. (B) RUNX1 ChIP results at the intron of ZNF238 and a control locus before (Control) and after (RUNX1) infection of the cells with empty or RUNX1-expressing retrovirus. (C) qPCR for ID genes in control and RUNX1-expressing RD cells. PCRs in the imaged gel in 3B were performed for the same number of cycles. The graph in 3B represents qPCR data showing the mean ± SEM of two independent experiments. Relative enrichment is calculated as the ratio of the % of input amplified with antibody to the % of input amplified with no antibody. qPCRs in 3A and 3C are represented as the mean ± SEM of at least three independent experiments. *: P < 0.05; **: P < 0.01.

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Skeletal Muscle

ISSN: 2044-5040

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