Figure 2
From: Inducible Cre transgenic mouse strain for skeletal muscle-specific gene targeting
Skeletal muscle-specific expression of MerCreMer (MCM) protein. Western blot analysis of different muscle and nonmuscle tissues from the human α-skeletal actin (HSA)-MCM transgenic strain detected the MCM protein (112 kDa) only in skeletal muscle (Esop, esophagus; Gstn, gastrocnemius; Pln, plantaris; Sol, soleus; EDL, extensor digitorum longus; EOM, extraocular muscle; Mastr, masseter; Tong, tongue; Quad, quadriceps; TA, tibialis anterior; Diaph, diaphragm) and not in the heart (Hrt), samples containing smooth muscle (Stom, stomach; S. Int, small intestine) or nonmuscle tissue (Lung; Panc, pancreas; Liver; Brain; Fat; Spln, spleen; Kdny, kidney). Both glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and tubulin, γ1 (TUBG1) were used as loading controls.