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Figure 4 | Skeletal Muscle

Figure 4

From: Measuring microRNA reporter activity in skeletal muscle using hydrodynamic limb vein injection of plasmid DNA combined with in vivo imaging

Figure 4

Quantification of miR-206 reporter activity in mdx4cv mice. (A) miR-206 expression measured in the gastrocnemius muscles of 3 month-old mdx4cv animals in comparison to C57Bl/6. miR-206 expression is normalized to sno202 ( n = 4 animals/group), mean values are displayed; error bars = standard error of the mean (SEM), *P ≤0.05. (B) Bioluminescent imaging of mdx4cv animals showing CBG99 luciferase and mKATE expression. Left hindlimbs were co-injected with pCBG99-2x-miR-206 and pcDNA-mKATE, and right hindlimbs were co-injected with pCBG99-Control and pcDNA-mKATE. (C) Region of interest (ROI) analysis of (B). The same region size was used for all animals (n= 4 animals/group; mean values are displayed; error bars = SEM, *P≤ 0.05.

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