FIG4 is expressed in skeletal muscle. Western blot analysis was performed to establish FIG4 expression using anti-FIG4 antibody. (A) Mouse multitissue Western blot reveals expression of FIG4 in a variety of tissues (Br = brain, Ht = heart, SmI = small intestine, Kid = kidney, Liv = liver, Lg = lung, SkM = skeletal muscle, St = stomach, Spl = spleen, Ov = ovary, Tes = testis). (B) FIG4 is expressed at multiple mouse ages in brain and skeletal muscle. Ages of mice tested were 1, 3, 6, 9 and 20 months. Left lanes are from brain, and right lanes are from quadriceps. The top blot was probed with anti-FIG4, and the bottom blot (loading control) was probed with glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (C) Western blot of protein extracts from C2C12 cells at various stages of differentiation. Differentiation was induced by serum withdrawal. Cells were differentiated until long myotubes were obviously present (day 8 = D8). (D) Analysis of wild-type littermate and pale tremor (plt) mouse skeletal muscle reveals that FIG4 is absent from plt muscle.