Figure 6

Human aRMS versus eRMS gene set differences are conserved in murine models when Rb1 is inactivated. (A) Principal component analysis (PCA) of mouse tumors using 12,370 genes that significantly discriminate among previously described murine eRMS [1] (red), Pax3:Foxo1a,p53 tumors (green) or Pax3:Foxo1a,p53,Rb1 tumors (blue), and normal skeletal muscle (black). Samples are colored according to their genotypes as indicated. Average log₂ intensity >6 and standard deviation >0.1 over 25 samples. Normal skeletal muscle (SkM) is shown as a control. Error bars represent the standard error of the mean. (B) PCA for differentially expressed genes between PAX-FOXO1A and fusion-negative RMS cell lines by Davicioni and colleagues [14]. (C) PCA for differentially expressed genes in fusion-positive aRMSaRMS versus fusion-negative eRMS by Laé and colleagues [19]. (D) PCA for differentially expressed F1/F2 metagenes by Williamson and colleagues [20]. (E) PCA for genes conserved in mouse and human Pax3:Foxo1a-positive aRMS by Nishijo and colleagues [11].