Skip to main content
Figure 3 | Skeletal Muscle

Figure 3

From: Immortalized myogenic cells from congenital muscular dystrophy type1A patients recapitulate aberrant caspase activation in pathogenesis: a new tool for MDC1A research

Figure 3

Caspase-3 activity was aberrantly activated in myotubes formed from immortalized MDC1A myoblasts. (A, B, C, D) Activated caspase-3 (green) was found in approximately 1% to 3% of the MyHC-expressing (red) differentiated cells in cultures of immortalized MDC1A cells. Two examples of 38/03-ct4 cultures are shown after 4 days in differentiation medium. (E) Arrows indicate nuclei with aberrant morphology that were in caspase-3-positive cells (caspase staining not shown). Nearby nuclei in caspase-3-negative cells showed normal morphology. Four fields are shown. (F) Quantification of caspase-3 enzymatic activity by two different assays showed that, after 4 days in differentiation medium, cultures of immortalized MDC1A cells (96/04-ct8 and 38/03-ct4) had significantly elevated levels of caspase-3 compared to cultures of immortalized healthy control cells (2/08-ct and 15Vbic-ct16) or immortalized FSHD (15Abic-ct24) cells. **P <001 (t-test, ‘n’ as shown).

Back to article page

Skeletal Muscle

ISSN: 2044-5040

Contact us