Immunoprecipitation analysis of a subset of interactions. A subset of interactions from the baits: abl-interactor 1 (ABI1), DYSF, SNAP-associated protein (SNAPIN) and telethonin (TCAP) was assessed using co-immunoprecipitation using myogenic cells or in gastrocnemius mouse muscle. Immunoblotting was performed with anti-prey antibody. Left lanes= input sample. Middle lanes: Protein lysates co-immunoprecipitated with anti-bait antibody. Right lanes= negative control where the primary antibody was omitted. One PPI for ABI1, 5 for DYSF, 1 for SNAPIN and 2 for TCAP were confirmed as positive by the co-immunoprecipitation assays.