Co-localization analyses of dysferlin and its partners. Double immunostaining for DYSF (Alexa488, green) and its Y2H partners (Alexa594, red) in cross human muscle cryosections by alphabetic order. The images were taken with a 40x objective for DES, FLNC, and NEB and a 63x for OPTN, KIF1B, DGKD, SNAPIN, and CMYA5. Scale bars = 20 μm. The Pearson’s coefficient is indicated under the merge image for each PPI. As expected, DYSF showed a membrane-associated pattern and a reticular cytoplasmic pattern corresponding to T-tubules on transversal section. Depending on the tested protein, the partner pattern is variable.