Altered mechanical characteristics induced by desmin mutants in tibialis anterior muscles. (A) Muscle specific maximal active force, (B) muscle fibrosis, (C) muscle weight and (D) absolute maximal active force were examined in WT (white bars), R406W (light grey bars) and E413K (dark grey bars) desmin expressing tibialis anterior muscles 1 month after AAV vectors injection. Note decrease in muscle specific maximal active force of R406W and E413K expressing muscles compared to WT (A). Asterisks indicate a significant difference compared to the WT (*P ≤0.05, **P ≤0.01, ***P ≤0.001). AAV, adeno-associated virus; WT, wild-type.