Figure 1
From: Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts
Activation of canonical Wnt signaling in embryonic, neonatal and adult skeletal muscles. Tcf-lacZ reporter mice were used to report activation of TCF promoter, the nuclear target of canonical Wnt signaling. X-gal staining (in blue) was used to reveal LacZ (b-gal) activity. (A) Whole mount staining of an E14.5 embryo. (B) Forelimbs and hind limbs at E14.5. (C) Ventral and (D) dorsal view of an E14.5 embryo revealing intensive staining in some muscles. (E) Hind limb and (F) forelimb at P0 (postnatal Day 0). (G) Diaphragm and (H) soleus muscles of adult mouse showing staining in a subset of myofibers with intensive signals at the neuromuscular junction area.