Figure 2

Activation of canonical Wnt signaling in adult slow myofibers. (A-D) Soleus myofibers from adult Tcf-lacZ mice were stained with antibodies for pan-myosin heavy chain (White) or slow myosin heavy chain (White), and β-gal (Red) and FITC conjugated BTX (Green). (E-F) Whole mount muscles co-stained with antibodies for slow (Blue) and fast (Red) myosin heavy together with beta-gal (Green) and BTX (Red). (G) Percentage of β-gal positive (n = 69) and negative (n = 60) myofibers that co-express slow myosin heavy chain.