Figure 4
From: Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts
Canonical Wnt signaling induces BMP-4 in C2C12 myoblasts. (A) qPCR analysis of the relative expression of various genes in C2C12 myoblasts overexpressing Wnt-1 and Wnt-3a under growth condition. (B) BMP-4 knockdown reduced the expression of alkaline phosphatase (Akp2) but not the Axin2 gene. (C-F) BMP-4 shRNA rescued the myogenic differentiation of Wnt-1 and Wnt-3a infected C2C12 cells. (C-D) Wnt-1 and Wnt-3a overexpressing myoblasts treated with Scramble shRNA; (E-F) Wnt-1 and Wnt-3a overexpressing myoblasts treated with BMP-4 shRNA. Black signaling is myosin heavy chain antibody staining reacted with 3, 3'-diaminobenzidine (DAB) substrate. (G) Western blotting showing myosin heavy chain protein expression after BMP-4 shRNA treatment. (H) qPCR analysis of myosin heavy chain gene expression. (I-L) BMP-4 antagonist Noggin-Fc dose-dependently rescued the myogenic differentiation of Wnt-3a infected C2C12 cells. The myosin heavy chain was labeled in black and nuclei were labeled in white.