Conditional inactivation of TSC1 in skeletal muscle. (A) Western blot analysis of soleus muscle from 90-day-old control (ctrl) and TSCmKO mice using antibodies directed against the proteins indicated. α-actinin is used as a loading control. (B) Weight of soleus (Sol), gastrocnemius (GC), plantaris (PL), tibialis anterior (TA), extensor digitorum longus (EDL) and triceps (Tri) muscles of TSCmKO and littermate control (ctrl) mice. Weight is expressed as a percentage of the weight of the same muscle in control mice after normalization to the total body weight (N = 8 to 12 mice for each genotype). Data are mean ± SEM; ***P <0.001; **P <0.01; *P <0.05; Student’s t-test. (C) H&E staining of cross-sections from TA and soleus muscles of control and TSCmKO mice. Scale bar = 50 μm. (D, E) Fiber size distribution in soleus (D) and TA (E) muscles of 90-day-old TSCmKO and control mice (N = 4). More details of fiber size analysis are shown in Additional file 1: Figure S3 and in Additional file 1, Table S1. *P <0.05.