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Table 1 Quantification of Western blot analysis

From: Differential response of skeletal muscles to mTORC1 signaling during atrophy and hypertrophy

  TSCmKO Ctrl Ratio Number of replicates
p-mTOR S2448 19 ± 2*** 10 ± 1 1.9 4
mTOR 31 ± 6 28 ± 5 1.1 4
P-4E-BP1 S65 23 ± 5*** 12 ± 3 1.9 4
4E-BP1 34 ± 6 39 ± 6 0.9 4
P-S6 S235/S236 53 ± 1*** 13 ± 6 4 4
S6 48 ± 11 42 ± 17 1.1 4
IRS-1 6 ± 2*** 24 ± 1 0.3 4
P-PKB/ Akt S473 4 ± 2*** 22 ± 3 0.2 4
PKB/ Akt 28 ± 7 22 ± 6 1.2 4
FoxO1 23 ± 5 18 ± 3 1.3 4
FoxO3a 16 ± 7 17 ± 5 0.9 4
P-FoxO1 T24 /3a T23 2 ± 7**/ 8 ± 3* 11 ± 2/ 20 ± 5 0.2/ 0.4 4
P-PKB/ AktS 473 (Den. TA) n.d. n.d. n.d. 3
P-S6 S235/S236 (Den. TA) 47 ± 12*** 5 ± 2 9.4 3
P-PKB/ Akt S473 (Den. Sol) n.d. n.d. n.d. 3
P-S6 S235/S236 (Den. Sol) 41 ± 10** 3 ± 5 13.7 3
  RAmKO Ctrl ratio number of replicates
P-PKB/ Akt S473 (Den. TA) 12 ± 2 2 ± 1 6 3
P-S6 S235/S236 (Den. TA) n.d. n.d. n.d. 3
P-PKB/ Akt S473 (Den. Sol) 19 ± 3 3 ± 2 6.3 3
P-S6 S235/S236 (Den. Sol) n.d. n.d. n.d. 3
  1. Proteins were extracted from soleus (Sol) and tibialis anterior (TA) muscles of 90-day-old TSCmKO, RAmKO or control (Ctrl) littermates. “Den.” denotes: denervated. “n.d” denotes: not detectable. The amount of total protein loaded onto the SDS-PAGE was adjusted and Western blots were additionally normalized to α-actinin levels. Numbers given represent average gray values ± SEM after subtraction of the background. “Ratio” represents the average gray value obtained from a knockout animal divided by the gray values from the control littermates. “Number of replicates” represents the number of knockout animals analyzed. The number of Ctrl littermates was always the same or higher than the values given. P-values were determined by Student’s t-test; * P <0.05, ** P <0.01, ***P <0.001.