Differential response of skeletal muscles to mTORC1 signaling during atrophy and hypertrophy

Table 1 Quantification of Western blot analysis

	TSCmKO	Ctrl	Ratio	Number of replicates
p-mTOR ^S2448	19 ± 2***	10 ± 1	1.9	4
mTOR	31 ± 6	28 ± 5	1.1	4
P-4E-BP1 ^S65	23 ± 5***	12 ± 3	1.9	4
4E-BP1	34 ± 6	39 ± 6	0.9	4
P-S6 ^S235/S236	53 ± 1***	13 ± 6	4	4
S6	48 ± 11	42 ± 17	1.1	4
IRS-1	6 ± 2***	24 ± 1	0.3	4
P-PKB/ Akt ^S473	4 ± 2***	22 ± 3	0.2	4
PKB/ Akt	28 ± 7	22 ± 6	1.2	4
FoxO1	23 ± 5	18 ± 3	1.3	4
FoxO3a	16 ± 7	17 ± 5	0.9	4
P-FoxO1 ^T24 /3a ^T23	2 ± 7*/ 8 ± 3	11 ± 2/ 20 ± 5	0.2/ 0.4	4
P-PKB/ AktS ⁴⁷³ (Den. TA)	n.d.	n.d.	n.d.	3
P-S6 ^S235/S236 (Den. TA)	47 ± 12***	5 ± 2	9.4	3
P-PKB/ Akt ^S473 (Den. Sol)	n.d.	n.d.	n.d.	3
P-S6 ^S235/S236 (Den. Sol)	41 ± 10**	3 ± 5	13.7	3
	RAmKO	Ctrl	ratio	number of replicates
P-PKB/ Akt ^S473 (Den. TA)	12 ± 2	2 ± 1	6	3
P-S6 ^S235/S236 (Den. TA)	n.d.	n.d.	n.d.	3
P-PKB/ Akt ^S473 (Den. Sol)	19 ± 3	3 ± 2	6.3	3
P-S6 ^S235/S236 (Den. Sol)	n.d.	n.d.	n.d.	3

Proteins were extracted from soleus (Sol) and tibialis anterior (TA) muscles of 90-day-old TSCmKO, RAmKO or control (Ctrl) littermates. “Den.” denotes: denervated. “n.d” denotes: not detectable. The amount of total protein loaded onto the SDS-PAGE was adjusted and Western blots were additionally normalized to α-actinin levels. Numbers given represent average gray values ± SEM after subtraction of the background. “Ratio” represents the average gray value obtained from a knockout animal divided by the gray values from the control littermates. “Number of replicates” represents the number of knockout animals analyzed. The number of Ctrl littermates was always the same or higher than the values given. P-values were determined by Student’s t-test; * P <0.05, ** P <0.01, ***P <0.001.