Figure 2
From: Myofiber branching rather than myofiber hyperplasia contributes to muscle hypertrophy in mdx mice
Innervation in the third EDL compartment. (A) The transgenic cyan fluorescence protein expression in neurons is pseudo-colored in green to demonstrate the innervation patterns in wild-type and mdx4cv muscles at 4 weeks of age. Note the profound infiltration of small acetylcholine receptor (AChR) patches in synaptic and non-synaptic regions within the mdx4cv muscles. Scale bar = 100 μm. (B) Shown are examples of ultra-terminal synapses (arrows) in 4-week-old muscle. Note that the ultra-terminal synapses are immature plaques. (C) Graph shows the mean +/- S.D. total number of ultra-terminal synapses demonstrating a modest reduction with age (P = 0.056; n = 4-6). (D) Shown is an example of denervation (arrow head) and non-synaptic ultra-terminal branching (arrow) in 20-week-old muscle. (E) The mean +/- S.D. total number of non-synaptic ultra-terminal branches increased with age (n = 4-6). **P <0.01 compared to 4 weeks of age. Scale bar for B and D = 30 μm.