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Figure 3 | Skeletal Muscle

Figure 3

From: Absence of γ-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle

Figure 3

Elevated p70S6K in γ-SG-/-muscles is independent of calcium. EDL muscles from C57 and γ-SG-/- mice were maintained in normal oxygenated Ringer’s solution, calcium-free oxygenated Ringer’s solution or oxygenated Ringer’s solution supplemented with tetracaine for 30 min. (A-D) Representative immunoblots and quantification for P-ERK1 (A), P-ERK2 (B), P-p70S6K (T389 site; C), and P-S6RP (D). Legend in A applies to all graphs. P-ERK 1 and 2 were normalized to T-ERK 1 and 2, respectively; P-p70S6K and P-S6RP were normalized to tubulin. Independent immunoblots were performed for each condition and γ-SG-/- activation levels were normalized to C57 activation levels in each case. n = 3 muscles per genotype and condition. Bars represent mean ± standard error. All datasets were tested by unpaired T test. * Significantly different from C57 by unpaired T test.

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Skeletal Muscle

ISSN: 2044-5040

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