Table 2 The oligonucleotide primers used for cloning the nebulin super repeats and for in vitro mutagenesis
From: Nebulin interactions with actin and tropomyosin are altered by disease-causing mutations
|  | Primer name | 5′-3′ Sequence | Experimental use |
|---|---|---|---|
1 | Ex 54-F | CGC GAA TTC CAA GGC TAC CGA AAG CAA | cDNA amplification |
2 | Ex 54-R | CCG CTC GAG ATC GCT CTG GAG GTC ATA GGC | cDNA amplification |
3 | Ex 78-F2 | TAC GGA TCC AAG TAC AAG GAA GGC TAC CG | cDNA amplification |
4 | Ex 78-R1 | TTT CTC GAG TGC TTG GAT AAT GTC GTT TTG | cDNA amplification |
5 | Ex 122-F1 | GCG GGA TCC GAG AAG CAG AAA GGT CAC TAC | cDNA amplification |
6 | Ex 122-R | CCG CTC GAG GAT GTT AAG CTT GCC AAC TCG | cDNA amplification |
7 | Ex 151-F | CGC GAA TTC AAA TTG GAA TAC AAC AAG GCC | cDNA amplification |
8 | Ex 151-R | CCG CTC GAG TTT GGC TGC CTG TGT GGC | cDNA amplification |
9 | Ex 54MUT-F | GATGGAGTCCCTTGGGTTCTTTAAAGGCAGAAAAGAAC | In vitro mutagenesis |
10 | Ex 54MUT-R | GTTCTTTTCTGCCTTTAAAGAACCCAAGGGACTCCATC | In vitro mutagenesis |
11 | Ex 78DEL-F | CATTACCGACACTCCGGAAATTGCCCTGACAATGAGCAAG | In vitro mutagenesis |
12 | Ex 78DEL-R | CTTGCTCATTGTCAGGGCAATTTCCGGAGTGTCGGTAATG | In vitro mutagenesis |
13 | Ex 122MUT-F | ACTGCTGTTCAGAGTGGCATTAATGCCATTGAGGTAAAATATAA | In vitro mutagenesis |
14 | Ex 122MUT R | TTATATTTTACCTCAATGGCATTAATGCCACTCTGAACAGCAGT | In vitro mutagenesis |
15 | Ex 151MUT-F | TTCACGTCAAGGAAGTGCCCAAGCATGTCAGTGAT | In vitro mutagenesis |
16 | Ex 151MUT R | ATCACTGACATGCTTGGGCACTTCCTTGACGTGAA | In vitro mutagenesis |
17 | Ex 78DEL-F | CATTACCGACACTCCGGAAATTGCCCTGACAATGAGCAAG | In vitro mutagenesis |
18 | Ex 78DEL-R | CTTGCTCATTGTCAGGGCAATTTCCGGAGTGTCGGTAATG | In vitro mutagenesis |