Figure 2

Insl6 muscle expression is increased in a murine model of myositis. Wild-type C57BL/6 female mice were immunized intradermally with 600 μg recombinant human skeletal muscle myosin-binding protein C or an equal volume of PBS (control) emulsified with Freund’s adjuvant. Two micrograms of pertussis toxin (PT) in PBS was injected intraperitoneally at the same time. (a) Gastrocnemius muscle from control and C protein-induced myositis (CIM) mice were harvested 14 days after immunization and analyzed by Western blotting for Insl6 and tubulin-α, as a loading control (left). The level of Insl6 was quantified relative to tubulin-α (right). (b) Transcript levels of Insl6 exon 1 and Neo cassette were measured 14 days after CIM induction in wild-type mice and Insl6-KO mice. The results are presented as mean ± SEM. ^*P < 0.05, †P < 0.01.