Skip to main content
Figure 3 | Skeletal Muscle

Figure 3

From: The injury-induced myokine insulin-like 6 is protective in experimental autoimmune myositis

Figure 3

Insl6-deficiency exacerbates experimental autoimmune myositis. Myosin-binding C protein fragment, Freund’s adjuvant and pertussis toxin (PT) were injected into homozygous Insl6-KO female mice and their wild-type littermates. (a) C protein-induced myositis (CIM) and control mice were examined on treadmill and Rotarod tests before and 14 days after the immunization. For the treadmill evaluation, a starting speed of six m/minute was set and increased by two m/minute every five minutes (left). The Rotarod test was performed at ten revolutions/minute for knockout (KO) and littermate wild-type mice. Running ability and Rotarod score were measured as described in Methods. (b) Representative image of tibialis anterior (TA) muscle sections stained with anti-CD4 and anti-CD8 eFluor® 650NC antibodies at day 14 after the immunization (left). The number of CD8 or CD4 cells per field was counted in 15 randomly selected fields. Quantitative data are presented as CD8 or CD4 cells per high-powered field (right). (c and d) Total RNA was isolated from lower limb muscles of control and CIM mice at post-immunized day 14, and cDNA was synthesized. Relative transcript expression of CD4, CD8, TNF-α and IFN-γ was measured by qRT-PCR. The data were normalized by 36B4 (n = 8, in each group). The results are presented as mean ± SEM. *P < 0.05, †P < 0.01.

Back to article page