Figure 4

Transgenic Insl6 over-expression ameliorates experimental autoimmune myositis. MCK-Insl6-TG female mice and wild-type littermates were subjected to the C protein-induced myositis (CIM) protocol. (a) Mice were examined on treadmill (left) and Rotarod (right) before and 14 days after the immunization. For the treadmill evaluation, a starting speed of ten m/minute was set and increased by five m/minute every five minutes. The Rotarod test was performed at 20 revolutions/minute for transgenic (TG) mice and wild-type littermates. Running distance and Rotarod score were measured as described in Methods. (b and c) Total RNA was isolated from lower limb muscles of control and CIM mice 14 days after immunization, and cDNA was synthesized. Relative transcript expression of CD4, CD8, TNF-α, and IFN-γ (n = 5 to 7 in each group) was measured by qRT-PCR. The data were normalized by 36B4. The results are presented as mean ± SEM. ^*P < 0.05, †P < 0.01.