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Figure 6 | Skeletal Muscle

Figure 6

From: The injury-induced myokine insulin-like 6 is protective in experimental autoimmune myositis

Figure 6

Insl6 inhibits Jurkat cell proliferation and IL-2 secretion induced by phorbol 12-myristate 13-acetate/phytohemagglutinin (PMA/PHA) stimulation. (a) Insl6 or β-gal encoding plasmid vectors were introduced into Jurkat T cells by electroporation. 3H-thymidine pulse labeling was performed 48 hours after the transfection (left). Data are expressed as relative to the mean of the β-gal experimental group (left). PMA (50 ng/ml) and PHA (1 μg/ml) were added to the culture medium 24 hours after the transfection. Cell culture medium was collected after 24 hours of PMA/PHA incubation. IL-2 concentration in the supernatant was determined by ELISA (right). (b) Differentiated C2C12 myotubes were transduced with Ad-β-gal or Ad-Insl6. Conditioned medium was harvested 48 hours after adenovirus transfection. Jurkat cells were incubated with this conditioned medium for 24 hours in the presence of 3H-thymidine. Incorporated 3H-thymidine was quantified. Results are presented as mean ± SEM.

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