NBD delivery improves the disease phenotype of the golden retriever model of Duchenne muscular dystrophy

Skeletal Muscle

Table 3 Composite analysis of histopathology markers in NBD-treated and untreated GRMD dogs

	Cranial sartorius	Lateral gastroc	Vastus lateralis	Diaphram	Average rescue
Inflammation	34	40	31	37	35.5
Necrosis (Foci,IgG)	25, 22 (p = 0.14)	34, 35	35, 32	37, 32	35.3, 30.3
Regeneration	43	35	44	41	40.8
			Final Histological Score of Improvement		35.5

Histological markers of inflammation, necrosis, and regeneration were quantitatively assessed from sections prepared from 4 muscle groups (cranial sartorius, lateral gastrocnemius, vastus lateralis, and diaphragm). Percent differences between GRMD and GRMD + NBD treated dogs were calculated and an average value for each histological marker was determined for each muscle group. A final composite average from each of these values were also calculated to represent the overall percent of improvement from NBD treatment with regards to reducing inflammation, necrosis, and regeneration. For all values except where indicated, p < 0.05.

ISSN: 2044-5040