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Figure 1 | Skeletal Muscle

Figure 1

From: Protein phosphatase 2C-alpha knockdown reduces angiotensin II-mediated skeletal muscle wasting via restoration of mitochondrial recycling and function

Figure 1

In vivo knockdown of PP2Cα. The indicated siRNAs were injected at five sites in Gas muscles which were then electroporated to induce uptake of the siRNA. The most effective sequence, time point, and dose of siRNA were chosen for subsequent AngII-infusion experiments. (A) Real-time PCR showing PP2Cα mRNA knockdown in Gas using 2.5 μg of four different PP2Cα siRNA target sequences relative to negative control siRNA at day 4 post electroporation. PP2Cα siRNA ‘A’ was used in all time course and dose response experiments. (B) Time-course of PP2Cα mRNA knockdown in Gas with 2.5 μg of scrambled and PP2Cα siRNA. (C) Time-course of PP2Cα protein knockdown in Gas with 2.5 μg of scrambled and PP2Cα siRNA. (D) Time-course of AMPK phosphorylation in Gas with 2.5 μg of scrambled and PP2Cα siRNA. (E) Dose response of PP2Cα mRNA knockdown in Gas at day 7 post electroporation. (F) Dose response of PP2Cα protein knockdown in Gas at day 7 post electroporation. (G) Dose response of AMPK phosphorylation in Gas at day 7 post electroporation. (H) Representative western blots showing PP2Cα protein knockdown and AMPK activation with 5 μg of PP2Cα siRNA at day 7. n =4-8 per group, Mean ± SEM, *P <0.05, **P <0.01, ***P <0.001.

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