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Figure 2 | Skeletal Muscle

Figure 2

From: Protein phosphatase 2C-alpha knockdown reduces angiotensin II-mediated skeletal muscle wasting via restoration of mitochondrial recycling and function

Figure 2

PP2Cα knockdown prevented AngII wasting. A total of 5 μg of the indicated siRNA sequences were injected at five sites in Gas muscles which were then electroporated to induce uptake of the siRNA. Three days later, osmotic minipumps were implanted subcutaneously and continually infused either saline or 1,000 ng/kg/min AngII for another 4 days. (A) Total body mass showing whole-body AngII wasting over 4 days of continuous infusion. (B) Gastrocnemius wet weight showing AngII muscle wasting and rescue with PP2Cα knockdown at 4 days. Two PP2Cα siRNA target sequences (A and B, Figure 1A) were tested to verify the specificity of the rescue effect. (C) Quadriceps wet weight showing AngII muscle wasting after 4 days. Quadriceps were not treated with siRNA, so quadriceps muscles ipsilateral to Gas receiving PP2Cα siRNA showed no rescue effect. (D) Average cross sectional area of Gas muscle fibers infused with either saline or AngII and treated with either scrambled or PP2Cα siRNA ‘A’. (E) CSA frequency distribution curves of Gas muscle fibers. n =6-32 per group, Mean ± SEM, **P <0.01, ***P <0.001, ****P <0.0001 (Saline scrambled vs. AngII scrambled), +P <0.05, +++P <0.001 (AngII scrambled vs. AngII PP2Cα siRNA).

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