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Figure 3 | Skeletal Muscle

Figure 3

From: Protein phosphatase 2C-alpha knockdown reduces angiotensin II-mediated skeletal muscle wasting via restoration of mitochondrial recycling and function

Figure 3

PP2Cα knockdown restored AMPK activation and signaling related to mitochondrial biogenesis. Specificity was ensured by performing experiments with two siRNA target sequences, as indicated. (A) Representative western blots showing effects of AngII and PP2Cα siRNA ‘A’ on the AMPK-PGC-1α-TFAM signaling axis. (B) PP2Cα protein expression was increased with AngII, which was prevented with PP2Cα siRNA. (C) Activating AMPK phosphorylation was reduced with AngII and increased with PP2Cα knockdown. (D) PGC-1α protein expression was reduced with AngII, which was prevented with PP2Cα siRNA. (E) PP2Cα siRNA increased expression of NRF1 transcription factor. (F) AngII reduced expression of the mitochondrial transcription factor TFAM, and this reduction was prevented by PP2Cα siRNA. n =4-32 per group, Mean ± SEM, *P <0.05, **P <0.01, ****P <0.0001 (Saline scrambled vs. AngII scrambled), +P <0.05, +++P <0.001 (AngII scrambled vs. AngII PP2Cα siRNA).

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Skeletal Muscle

ISSN: 2044-5040

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