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Figure 5 | Skeletal Muscle

Figure 5

From: Protein phosphatase 2C-alpha knockdown reduces angiotensin II-mediated skeletal muscle wasting via restoration of mitochondrial recycling and function

Figure 5

PP2Cα knockdown increased mitochondrial content and partially restored AngII-induced mitochondrial dysfunction. All data were generated from experiments utilizing PP2Cα siRNA ‘A’. (A) UCP-3 expression, (B) NADH dehydrogenase (complex I) expression, (C) succinate dehydrogenase (complex II) expression, (D) cytochrome bc1 complex (complex III) expression, and (E) cytochrome C oxidase (complex IV) expression were not altered by AngII or PP2Cα siRNA. (F) AngII reduced ATP synthase (complex V) expression, which was not restored with PP2Cα siRNA. (G) Relative mitochondrial copy number was unaltered by AngII, but PP2Cα siRNA increased mitochondrial load. (H) Complex IV (Cytochrome C Oxidase) activity was reduced with AngII and partially restored with PP2Cα knockdown. (I) ATP was reduced with AngII and partially restored with PP2Cα knockdown. (J) Representative western blots showing expression of ETC., complexes I-V, and uncoupling protein-3. n =6-20, Mean ± SEM, ***P <0.001 (Saline scrambled vs. AngII scrambled), +P <0.05 (AngII scrambled vs. AngII PP2Cα siRNA).

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Skeletal Muscle

ISSN: 2044-5040

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