Figure 8

AngII inhibited expression of mitochondrial fission and fusion proteins via a MARCH5-independent mechanism. All data were generated from experiments utilizing PP2Cα siRNA ‘A’. (A) Representative western blots showing effects of AngII and PP2Cα siRNA on markers of mitochondrial fusion and fission. (B) AngII reduced Mitofusin-2 expression, which was not prevented by PP2Cα knockdown. (C) AngII reduced OPA1 expression, which was not prevented by PP2Cα knockdown. (D) AngII reduced Fis1 expression, which was not prevented by PP2Cα knockdown. (E) S616 phosphorylation of DRP1 was increased with PP2Cα siRNA, which facilitates mitochondrial fission. (F) DRP1 expression was reduced by AngII, and this was not rescued by PP2Cα knockdown. (G) Mff expression was not altered by AngII or PP2Cα siRNA. (H) MARCH5 expression was not induced with AngII and was unchanged by PP2Cα siRNA. n =4-20, Mean ± SEM, *P <0.05, **P <0.01, ***P <0.001 (Saline scrambled vs. AngII scrambled), +P <0.05 (AngII scrambled vs. AngII PP2Cα siRNA).