Figure 3
From: SMASH – semi-automatic muscle analysis using segmentation of histology: a MATLAB application
Segmentation examples. Example muscle section of 1-year-old soleus muscle from mdx mouse. Red is laminin stain, green is slow muscle myosin heavy chain, and blue is DAPI. (A) Segmentation filter value of 2 produces over segmentation of original image. (B) Segmentation filter value of 5 produces appropriate segmentation. (C) Segmentation filter value of 12 produces under segmentation of original image. (D) Reducing the brightness of laminin staining by 50% (representing poor staining) produces under segmentation. (E) Enhancing the brightness of laminin by 400% (representing over exposure) produces over segmentation. (F) Overexposure can be compensated for by increasing the segmentation filter value to 12. Yellow arrow heads show areas of over segmentation. Pink arrow heads show areas of under segmentation.