Centrally nucleated fibers. (A) Input box with options for CNF function. (B) Truncated example of Excel output from running CNF function. (C) Portion of immunofluorescent image. Red is laminin stain, green is slow muscle myosin heavy chain, and blue is DAPI (top left). Image showing filtered nuclei (top right). Image with boarder regions in red and nuclei above threshold in blue (bottom left). Image in which only CNFs are depicted in white (bottom right).