Figure 7
From: Novel and optimized strategies for inducing fibrosis in vivo: focus on Duchenne Muscular Dystrophy
Fibrosis induction in muscle of wild-type mice after repeated BaCl 2 injuries. (A) Sirius red, H&E and fibronectin staining on wild-type (WT) tibialis anterior (TA) muscles subjected to one or six consecutive weekly rounds of BaCl2 injections (50 μl of 0.2% BaCl2), compared to non-injured (NI) muscle of sham-operated WT mice, sampled two weeks after the final injection. (B) Quantitative expression of fibronectin and collagen I in the distinct muscle samples (values are means ± SEM; n = 4 to 5 on each group. Non-parametric Mann–Whitney U test; **P <0.05 versus NI). (C) Ex vivo maximum isometric force (tetanic force) of TA muscle. Values as mean ± SEM; n = 4 for each group; non-parametric Mann–Whitney U test; no significant differences P >0.05. Scale bar = 50 μm.