Figure 10

No differences in differentiation of muscle progenitor cells in vitro . Proliferating muscle stem cells isolated from hind limb muscles of wildtype (WT) and miR-206/133b knock-out mice (KO) were induced to differentiate and stained after 3 or 5 days in differentiation medium (DM) for expression of myosin (green: MF20 staining, blue: nuclear staining; the scale bar corresponds to 50 μm). No differences in cell morphology or differences in the expression of myosin were detected between WT and mutant cells (A). Formation of fused myotubes from differentiating myoblasts was not impaired by the loss of the miR-206/133b cluster (B). Pax7 expression in differentiating myogenic progenitor cells was detected by western blot. No differences in Pax7 expression were detected after loss of miR-206/133b (C).