Activity-dependent CaMKII translocation and activation. (A) KN-93 decreases the height of the Ca2+ transient during repetitive stimulation. Tetanic calcium response averaged from first to 50th peak (50 pulses for one second) and the calculated averaged response was plotted as in Figure 2B. (B) Ratio of p-CaMKII to CaMKII in muscles of EK and WT mice (%WT). Inset: Representative western blot of p-CaMKII and CaMKII. (C) Representative immunocytochemistry images showing co-localization of CaMKII and CaV1.1 in single WT and EK FDB fibers. (D and E) Representative line profiles of immunofluorescence for CaMKII and CaV1.1 in (D) WT and (E) EK FDB fibers. (F) Representative images for the PLA assay confirming a close association of CaMKII with CaV1.1. Scale bar = 20 μm. For negative control (right), normal rabbit IgG was used instead of CaMKII antibody. (G) Analysis of average spot density in the proximity ligation assay in fiber resting and electrically stimulated. Spots are analyzed in fibers from three mice of each genotype. (H) Effect of AIP on the amplitude of the Ca2+ transient with repetitive stimulation. (I) 4CmC-induced Ca2+ release post stimulation in the presence and absence of AIP. (J) Changes associated with the EK mutation in CaV1.1. Values are shown as mean ± SEM. *P <0.05, **P < 0.01, and ***P < 0.001.