Figure 1

Expression of Galgt1 during cardiotoxin-induced muscle regeneration. (A) Galgt1 signal data from the publicly available Children’s National Medical Center Affymetrix microarray study on regenerating mouse muscle after cardiotoxin-induced injury. (B) qRT-PCR measurement of the fold change in the expression of Galgt1 gene in WT muscle 1, 4, 7, 14, or 28 days after cardiotoxin-induced muscle injury. (C) Wild type gastrocnemius muscle was stained with antibody to Galgt1-dependent complex ganglioside GM1, Galgt1 protein, or with secondary antibody alone (control) after cardiotoxin-induced injury of wild type muscle. Errors in (B) are SEM averaged from six muscles per condition with two to three measures per muscle. ***P < 0.001, Bar is 50 μm.