gene expression is elevated in muscle cells during muscle regeneration. (A) Immunostaining with antibodies to GM1 (red) and antibodies to cell-specific markers (green) for muscle cells (integrin α7), immune cells (CD11b), fibroblasts (Ertr7), or mesenchymal stem cells (Sca1), with DAPI (blue) nuclear stain, 1 day after cardiotoxin injection into the gastrocnemius muscle. Three-color merged images are shown on the right. (B) qRT-PCR comparison of Galgt1 gene expression in FACS-sorted cell populations after 1 day of cardiotoxin-induced injury compared to the same populations sorted from untreated controls. ***P < 0.001 (comparing CD31−CD45−integrin α7+ to any of the other shown conditions). Errors in (B) are SEM averaged from four experiments per condition with two to three measures per muscle. Bar is 50 μm.