Altered differentiation of cultured
muscle cells. (A) Phase-contrast images of cultured confluent wild type (WT) and Galgt1-deficient (Galgt1
−/−) primary muscle cells 6 days after placement in myofiber differentiation media. (B) Area of myofibers per unit area of the visual field at 1, 3, or 6 days after addition of differentiation media. (C) Number of myofibers present per unit area of the field of view at 1, 3, or 6 days after addition of differentiation media. (D) Growth rate of isolated primary muscle cells cultured in growth media. Errors are SEM for n = 6 experiments in (B)–(D), with three to six replicates per data point. *P < 0.01, ***P < 0.001, Bar is 50 μm.