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Table 1 Comparison of changes in RyR1 channel gating parameters induced by association of CSQ1 constructs

From: C-terminal residues of skeletal muscle calsequestrin are essential for calcium binding and for skeletal ryanodine receptor inhibition

  

( X  ± SEM)

  

WT CSQ

CSQ1 DA1

CSQ1 DA2

CSQ1 DA3

CSQ1 ΔC7

CSQ1 ΔC14

RyR1-CSQ

P o

0.19 ± 0.02

0.19 ± 0.03

0.17 ± 0.07

0.15 ± 0.04

0.12 ± 0.01

0.12 ± 0.02

RyR1 + CSQ

P o

0.08 ± 0.01*

0.07 ± 0.01*

0.06 ± 0.02*

0.07 ± 0.01*

0.10 ± 0.01*#

0.15 ± 0.01*#

RyR1-CSQ

T o

4.93 ± 1.40

3.55 ± 0.26

3.44 ± 0.28

3.08 ± 0.42

3.80 ± 0.48

7.09 ± 1.99

RyR1 + CSQ

T o

2.43 ± 0.53*

1.96 ± 0.15*

2.78 ± 0.23*

1.99 ± 0.36*

2.67 ± 0.30*

6.70 ± 1.76#

RyR1-CSQ

T c

18.73 ± 4.02

25.03 ± 4.95

22.20 ± 6.40

33.85 ± 5.37

115.78 ± 38.29#

40.62 ± 7.81#

RyR1 + CSQ

T c

33.41 ± 4.97*

39.74 ± 10.72*

38.01 ± 5.78*

46.14 ± 5.86#*

115.69 ± 50.11#

36.95 ± 10.40

RyR1-CSQ

F o

34.15 ± 2.72

39.68 ± 3.27

41.78 ± 5.65

32.97 ± 5.95

16.9 ± 4.50#

26.18 ± 7.95

RyR1 + CSQ

F o

27.32 ± 5.66

36.37 ± 8.20

39.20 ± 7.20

27.76 ± 5.79

17.46 ± 4.40#

25.18 ± 6.49

  1. Endogenous CSQ1 was first dissociated from RyR1. The first row for each parameter shows average data for channel activity following dissociation of endogenous CSQ1 (RyR1-CSQ1) and before addition of the recombinant CSQ1 construct. The second row in each parameter shows average data after addition of the indicated construct. Parameters are open probability (P o ), open time (T o ), closed time (T c ), and open frequency (F o ), which are the mean ± SE (N = 7 to 12) of combined data recorded at +40 and −40 mV. Asterisks (*) indicate average parameter significantly different (P ≤ 0.05) from that recorded in the absence of CSQ1, and crosshatch (#) indicates average parameter significantly different (P ≤ 0.05) from that recorded after addition of WT CSQ1.
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Skeletal Muscle

ISSN: 2044-5040

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