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Figure 1 | Skeletal Muscle

Figure 1

From: Myocyte enhancer factor 2C function in skeletal muscle is required for normal growth and glucose metabolism in mice

Figure 1

Generation of skeletal muscle-specific Mef2c knockout mice. (A) Schematic depicting the strategy for deleting Mef2c from skeletal muscle. Mef2c-73k-CreTg/0; Mef2c +/− males were crossed to Mef2c flox/flox female mice to generate control (Mef2c flox/+) and Mef2c skeletal muscle knockout mice (Mef2c SkMKO), which have the genotype Mef2c-73k-CreTg/0; Mef2c flox/− (shown in red text). (B) All genotypes were observed at expected Mendelian ratios (χ 2 = 0.328, 3 d.f.). (C-F) Mef2c was readily detected in somites of control mice at E9.5 (C) and in skeletal muscles of control mice at E13.5 (E). In contrast, Mef2c transcripts were not detected by in situ hybridization in somites at E9.5 (D) or in skeletal muscles at E13.5 (F) in Mef2c SkMKO mice. (G) Semi-quantitative RT-PCR analyses of three control and three Mef2c SkMKO adult quadriceps muscle detected Mef2c transcripts in control but not in Mef2c SkMKO. L7 transcripts were similarly detected in samples from all six mice. (H) Expression of Mef2c in adult quadriceps muscle was analyzed by quantitative real-time RT-PCR (qPCR). Mef2c SkMKO mice showed more than 95% reduction in Mef2c transcripts compared to control muscle (n = 3; **p < 0.01). Values shown are the mean + standard deviation.

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