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Figure 3 | Skeletal Muscle

Figure 3

From: Myocyte enhancer factor 2C function in skeletal muscle is required for normal growth and glucose metabolism in mice

Figure 3

Mef2c is required for normal fiber type composition in skeletal muscle. Soleus muscle sections from control (ctl) and Mef2c SkMKO (cko) male mice at 52 days of age were analyzed for morphology and fiber type. (A, B) Hematoxylin and eosin stain for morphological analysis on control and Mef2c SkMKO muscle sections at 10× magnification showed no overt differences in skeletal muscle morphology. (C, D) Immunofluorescence staining for fast myosin (MY32) for fiber type identification from control and Mef2c SkMKO muscle sections at 10× magnification showed a greater percentage of fast fibers in Mef2c SkMKO muscles than in controls. Red staining marks fast/type II fibers; slow/type I fibers are unstained. (E) Quantification of slow/type I fibers as detected by MY32 immunofluorescence staining in control and Mef2c SkMKO muscle sections showed a significant decrease in the number of slow fibers in Mef2c SkMKO mice (25.4% ± 1.2% slow) compared to control mice (32.8% ± 0.8% slow); n = 8; ***p < 0.001. Values shown are the mean + standard deviation.

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