Figure 3

TGFβ treatment stimulates C/EBPβ expression in myoblasts. (A) Western analysis of C/EBPβ and Pax7 expression in C2C12 myoblasts cultured in differentiation medium in the presence or absence of TGFβ for 96 h. Actin is used as a loading control. (B) Quantification of C/EBPβ expression from western blots in panel (A) relative to vehicle-treated controls. Error bars are the SEM, n = 3, *P < 0.05. (C) Quantification of Pax7 expression from western blots from (A) relative to vehicle-treated controls. Error bars are the SEM, n = 3, **P < 0.01. (D) C/EBPβ protein expression after 96 h in differentiation medium (DM) in the continual presence of TGFβ and/or RA, as indicated. Actin is used as a loading control. (E) Quantification of western blots from (D) relative to the vehicle-treated controls. Error bars represent the SEM. Means marked with different letters are statistically different, meeting a minimum cutoff of P < 0.05, n = 4. (F) Cebpb mRNA expression in C2C12 myoblasts induced to differentiate in the presence or absence of TGFβ and RA for 96 h. Data is shown relative to vehicle-treated controls, and error bars are the SEM, n = 3. Means marked by different letters are statistically different from one another, meeting the minimum cutoff of P < 0.05.