Figure 10

Elevated PGC-1α results in increased TFEB protein levels. (A-B) Blots and quantification TFEB protein level in TA muscle and in nuclear fraction of wild type (WT) and PGC-1α Tg (Tg) control (Con) and denervated (Den). (A) Representative blots. (B) Quantification of TFEB protein in TA muscle. *P < 0.05 significant difference between Con and Den. †P < 0.05 significant effect of genotype. GAPDH was used as loading control for whole muscle and histone 2 B (H2B) was used as nuclear loading control (n = 3 to 5 for all groups). GAPDH, glyceraldehyde 3-phosphate dehydrogenase. H2B, histone 2 B; Nuc. TFEB, nuclear transcription factor EB; TFEB, transcription factor EB.