Figure 4

Lack of PGC-1α results in attenuated mitophagy flux. (A-D) Blots and quantification of autophagic proteins in isolated mitochondrial fractions in control (Con), denervated (Den) WT, and PGC-1α KO (KO) animals treated with vehicle (water) or colchicine (col) 0.4mg/kg/day for 4 days. (A) Representative blots. Quantification of (B) LC3BII and (C) p62. (D) Representative blots. Quantification of mitochondrial (E) ubiquitin, (F) parkin, (G) basal mitophagy flux, and (H) denervation-induced mitophagy flux. (I) Confocal images of fixed single fibers immuno-stained for cytochrome c (as a mitochondrial marker, green) and lysosomal Lamp-2 (red); their colocalization (yellow) represents mitochondria within lysosomes. *P < 0.05 significant difference between Con and Den. †P < 0.05 significant effect of genotype. VDAC was used as loading control (n = 3 to 5 for all groups). AU, arbitrary units; KO, knockout; VDAC, voltage-dependent anion channel; WT, wild type.