Figure 5

Lack of PGC-1α results in lower TFEB protein levels and nuclear localization. (A-C) Blots and quantification TFEB protein levels between wild type (WT) and PGC-1α KO (KO) control (Con) and denervated (Den). (A) Representative blots. Quantification of (B) whole muscle TFEB protein. (C) Nuclear localization of TFEB in TA muscle. *P < 0.05 significant difference between Con and Den. †P < 0.05 significant effect of genotype. GAPDH was used as loading control for whole muscle, and histone 2 B (H2B) was used as nuclear loading control (n = 4 to 8 for all groups). GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Nuc. H2B, nuclear histone 2B; Nuc. TFEB, nuclear transcription factor EB; TFEB, nuclear transcription factor EB.